Bromelain Activity of Waste Parts of Two Pineapple Varieties

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Isolation and Purification of Bromelain from Waste Peel of Pineapple for Therapeutic Application

The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, mainta...

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Thermal stability of free bromelain and bromelain-polyphenol complex in pineapple juice

Pineapple (Ananas comosus), Bromeliaceae family, is a fruit grows in tropical countries including Malaysia. This fruit has several pharmacological benefits due to the presence of high concentration of bromelain (cysteine proteases). Condition of elevated temperature will induce deformation of enzyme and result in loss of activity. Sulfhydryl groups in cysteine proteases are readily to be oxidiz...

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Effect of salt stress on the activity of bromelain in pineapple plants grown In vitro

Background Bromelain is a collective name for proteolytic enzymes or proteases (natural proteolytic enzymatic complex) found in tissues including stems, fruit and leaves of the pineapple (Ananas comosus var. comosus) and of other plant species of the family Bromeliaceae [1]. Is a bioactive agent possessing remarkable therapeutic properties such as reversible inhibition of platelet aggregation, ...

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Aroma Volatile Compounds from Two Fresh Pineapple Varieties in China

Volatile compounds from two pineapples varieties (Tainong No.4 and No.6) were isolated by headspace solid phase microextraction (HS-SPME) and identified and quantified by gas chromatography-mass spectrometry (GC/MS). In the Tainong No. 4 and No. 6 pineapples, a total of 11 and 28 volatile compounds were identified according to their retention time on capillary columns and their mass spectra, an...

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Partitioning of bromelain from pineapple peel (Nang Lae cultv.) by aqueous two phase system

Pineapple peel is a potential source for the extraction of beneficial bioactive compounds due to the large amount of waste after processing. The extraction of bromelain from pineapple peel by using aqueous two-phase system (ATPs) and its application in muscle models were investigated. Bromelain predominantly partitions to the polyethylene glycol (PEG) rich top phase. The highest enzyme activity...

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ژورنال

عنوان ژورنال: Sustainable Food Production

سال: 2018

ISSN: 2624-876X

DOI: 10.18052/www.scipress.com/sfp.2.21